if i have only one sample, how can i say if a gene expression are upregulated significantly or not? how can i set a standard? using some public data to get an average level of gene expression or set a reference gene level,like actin? -- shan gao Room 231(Dr.Fei lab) Boyce Thompson Institute for Plant Research Cornell University Tower Road, Ithaca, NY 14853-1801 Office phone: 1-607-254-1267(day) Official email:sg839@cornell.edu Facebook:http://www.facebook.com/profile.php?id=100001986532253 [[alternative HTML version deleted]]

Hi guys, I tried to install gmapR in R3.0.0 in windows 7, but kept having troubles: > biocLite("gmapR") BioC_mirror: http://bioconductor.org Using Bioconductor version 2.12 (BiocInstaller 1.9.11), R version 3.0.0. Installing package(s) 'gmapR' package ‘gmapR’ is available as a source package but not as a binaryWarning message: package ‘gmapR’ is not available (for R version 3.0.0) > > biocLite("gmapR",type="source") BioC_mirror: http://bioconductor.org Using Bioconductor version 2.12 (BiocInstaller 1.9.11), R version 3.0.0. Installing package(s) 'gmapR' trying URL 'http://bioconductor.org/packages/2.12/bioc/src/contrib/gma pR_1.1.16.tar.gz' Content type 'application/x-gzip' length 4473453 bytes (4.3 Mb) opened URL downloaded 4.3 Mb* installing *source* package 'gmapR' ... ** libs*** arch - i386 ERROR: compilation failed for package 'gmapR' * removing 'C:/Program Files/R/R-3.0.0/library/gmapR'The downloaded source packages are in ‘C:\Users\Administrator\AppData\Local\Temp\Rtmp2nMJFF\download ed_packages’ Warning messages: 1: running command '"C:/PROGRA~1/R/R-30~1.0/bin/x64/R" CMD INSTALL -l "C:\Program Files\R\R-3.0.0\library" C:\Users\ADMINI~1\AppData\Local\T emp\Rtmp2nMJFF/downloaded_packages/gmapR_1.1.16.tar.gz' had status 1 2: In install.packages(pkgs = pkgs, lib = lib, repos = repos, ...) : installation of package ‘gmapR’ had non-zero exit status > > install.packages("gmapR_1.1.16.tar.gz",repos=NULL,type="source") * installing *source* package 'gmapR' ... ** libs*** arch - i386 ERROR: compilation failed for package 'gmapR' * removing 'C:/Program Files/R/R-3.0.0/library/gmapR' Warning messages: 1: running command '"C:/PROGRA~1/R/R-30~1.0/bin/x64/R" CMD INSTALL -l "C:\Program Files\R\R-3.0.0\library" "gmapR_1.1.16.tar.gz"' had status 1 2: In install.packages("gmapR_1.1.16.tar.gz", repos = NULL, type = "source") : installation of package ‘gmapR_1.1.16.tar.gz’ had non-zero exit status Does gmapR work in windows? Thanks, Ying > sessionInfo() R version 3.0.0 (2013-04-03) Platform: x86_64-w64-mingw32/x64 (64-bit)locale: [1] LC_COLLATE=English_United States.1252 LC_CTYPE=English_United States.1252 [3] LC_MONETARY=English_United States.1252 LC_NUMERIC=C [5] LC_TIME=English_United States.1252 attached base packages: [1] stats graphics grDevices utils datasets methods base other attached packages: [1] BiocInstaller_1.9.11loaded via a namespace (and not attached): [1] tools_3.0.0 > [[alternative HTML version deleted]]

thank you very much for your information it is useful, although i mean a RNA-seq data shan [[alternative HTML version deleted]]