We are running experiments where there are technical difficulties in getting a pure template of interest. What we have is a mix of template-1 (our template of interest) and template-2 (the contaminating template). We have pure samples of template-2 that we run alongside the mix. We also run several HSK genes to use for normalization. Using HTqPCR, is there a way to remove the signal in the mix due to template-2 ? Thanks. Susanne [[alternative HTML version deleted]]