Hi- I have 6GB and 7GB bam files that I am using with the DEXSeq analysis. The size of these files makes it impossible for me to open them up and see whats inside. I mention this because I have a feeling that I need to know the contents in order to set up the "sample" data frame from the pasilla vignette. I tried sample =data.frame("file.bam") but when I enter sample, I get the file name in return and nothing else. Any tips on how to get my sample to look similar to table 1 in the pasilla vignette? Thanks, ML ASU Grad Student [[alternative HTML version deleted]]