i got 70458 transcripts of one worm by de novo assembly, but i found 11825 of them are Escherichia contamination. i think if i donot remove them,it will bother the later analysis, e.g. normalization ?? -- shan gao Room 231(Dr.Fei lab) Boyce Thompson Institute for Plant Research Cornell University Tower Road, Ithaca, NY 14853-1801 Office phone: 1-607-254-1267(day) Official email:sg839@cornell.edu Facebook:http://www.facebook.com/profile.php?id=100001986532253 [[alternative HTML version deleted]]