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Yanxiang Shi
▴
40
@yanxiang-shi-5992
Last seen 10.3 years ago
Hi,
I am planning to do RNA-seq on bacterial samples collected at
different
time points after treatment. I am just wondering, what should be the
control of this series of samples, should it be untreated sample at
time 0
right before the treatment, or should it be untreated samples at
corresponding time points? As the RNA-seq cost a good amount of money,
I
think "untreated samples at different corresponding time points" would
cost
a lot, but which way would make more sense both biologically and
statistically? I will do triplicates for each time point and
condition.
I am new to this field so get really confused some time. Thank you
very
much in advance!!!
Best,
Nancy
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