All,
I'm using R 1.9.0 in Win2000 with a fresh install of R and
Bioconductor via
getBioC.
When I run the help file example for pm.getabst:
library(annotate)
library(hgu95av2)
hoxa9 <- "37809_at"
absts <- pm.getabst(hoxa9, "hgu95av2")
it runs for a minute, then either stops R or indefinitely hangs
(depending
on which computer I use). I haven't seen any error messages.
I have also make sure that I've started R using the --internet2
option.
Any thoughts? Thanks,
Max
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Robert,
Thanks for the reply. The internet connection was my first thought.
The
fresh install I did after first noticing the problem was done through
R via
install.packages and getBioC, so I'd be surprised if that's the issue
(but
this is windows...). Without using the --internet2 flag,
install.packaes
will fail with a message, but this seams like different behavior.
I don't usually hit NCBI databases and I can get to web pages via
output
from other annotation functions.
Also, I did not get to the point where R prints that the XML package
was
loaded. Given this, I just put a browser() into pubmed and it seems to
crash when
return(.handleXML(query))
is executed. Given this, I'll cc the list on this message.
Max
|---------+---------------------------->
| | Robert Gentleman |
| | <rgentlem@jimmy.h| |="" |="" arvard.edu=""> |
| | |
| | 07/23/2004 09:18 |
| | AM |
| | |
|---------+---------------------------->
>-------------------------------------------------------------------
--------------------------------------------|
|
|
| To: Max_Kuhn@bd.com
|
| cc:
|
| Subject: Re: [BioC] problems with pm.getabst
|
>-------------------------------------------------------------------
--------------------------------------------|
With a slightly newer version of all, I get:
> absts <- pm.getabst(hoxa9, "hgu95av2")
Loading required package: XML
> absts
$"37809_at"
$"37809_at"[[1]]
An object of class 'pubMedAbst':
Title: Aberrant expression of HOXA9, DEK, CBL and CSF1R in acute
myeloid leukemia.
PMID: 14738146
Authors: S Casas, B Nagy, E Elonen, A Avent
On, ML Larramendy, J Sierra, T Ruutu, S Knuutila
Journal: Leuk Lymphoma
Date: Nov 2003
Are you sure you have an internet connection at all?
you could try downloading a package using install.packages to see.
Also, and if so, you may not be able to get stuff from NCBI - they do
ban IPs if they get too many requests
but I am reasonably sure your problems are due to internet
connectivity (and you if fresh installing let me suggest a more
recent version of R - I think 1.9.1 is the latest stable)
Robert
+---------------------------------------------------------------------
------+
| Robert Gentleman phone : (617) 632-5250
|
| Associate Professor fax: (617) 632-2444
|
| Department of Biostatistics office: M1B20
|
| Harvard School of Public Health email: rgentlem@jimmy.harvard.edu
|
+---------------------------------------------------------------------
------+
-----------------------------------------
This message is intended only for the designated
recipient(s...{{dropped}}
Dear users,
I am working with 14 U133plus chips. I read in my data using
ReadAffy()
and it was a bit slow but worked fine after having increased the
memory
usage to 3000.
I have tried to obtain some degradation plots and this time the
computer crashes. Is AffyRNADeg that demanding?
>Raw.Data <- ReadAffy()
>deg <- AffyRNADeg(Raw.Data)
I am running R 1.9.1 on a PC, 512megas RAM
Also, how could I label the outcome lines of plotAffyRNAdeg so that I
graphically know which chip is the odd one in the case there is one?
Maybe use the "legend" function, but how?
Thanks for your help
Regards
David