Hi, I am using Limma for a non-affy single channel dataset. the design is a 2X2 factorial design, but one chip is missing. array gender genotype 1 M mutant 2 M mutant 3 F mutant 4 F mutant 5 M wild 6 M wild 7 F wild How to make the design matrix? Thank you very much. Yihuan

At 06:04 AM 27/07/2004, Yihuan Xu wrote: >Hi, > >I am using Limma for a non-affy single channel dataset. the design is a 2X2 >factorial design, but one chip is missing. > >array gender genotype >1 M mutant >2 M mutant >3 F mutant >4 F mutant >5 M wild >6 M wild >7 F wild > >How to make the design matrix? Why not design <- model.matrix(~factor(gender)*factor(genotype),data=targets) where 'targets' is your targets data.frame? Gordon >Thank you very much. > >Yihuan