Saving all AffyPLM residual plots to disk
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lep ▴ 90
@lep-6139
Last seen 9.3 years ago
United States
We are interested in saving all the AffyPLM residual plots to disk (in a single folder) but cannot find syntax in the user's guide or in forums on how to do this. Thx ahead of time, LP Houston Methodist. Leading Medicine. Ranked by U.S.News & World Report as one of America's "Best Hospitals" in 13 specialties. Named to FORTUNE? Magazine's "100 Best Companies to Work For?" list eight years in a row. Designated as a Magnet hospital for excellence in nursing. Visit us at houstonmethodist.org. Follow us at twitter.com/MethodistHosp and www.facebook.com/HoustonMethodist ***CONFIDENTIALITY NOTICE*** This e-mail is the property of Houston Methodist Hospital and/or its relevant affiliates and may contain restricted and privileged material for the sole use of the intended recipient(s). Any review, use, distribution or disclosure by others is strictly prohibited. If you are not the intended recipient (or authorized to receive for the recipient), please contact the sender and delete all copies of the message. Thank you.
affyPLM affyPLM • 1000 views
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@james-w-macdonald-5106
Last seen 11 hours ago
United States
Hi Leif, Here is some code I use to do this. Here I assume you have both an ExpressionSet called 'eset' and a PLMset called 'plm'. I usually use oligo, so in my case it is an oligoPLM object, but no matter. sn <- gsub("\\.CEL", "", sampleNames(eset)) ## don't re-make existing plots if(!all(file.exists(paste(sn[length(sn)], "png", sep = ".")))) { sn <- sn[!file.exists(paste(sn[length(sn)], "png", sep = "."))] for(i in seq_len(length(sn))){ png(paste0(sn[i], "png")) image(plm, type = "residuals", which = i) dev.off() } } Best, Jim On Tuesday, October 01, 2013 1:43:37 PM, Peterson, Leif wrote: > We are interested in saving all the AffyPLM residual plots to disk (in a single folder) but cannot find syntax in the user's guide or in forums on how to do this. Thx ahead of time, LP > > Houston Methodist. Leading Medicine. > > Ranked by U.S.News & World Report as one of America's "Best Hospitals" in 13 specialties. Named to FORTUNE? Magazine's "100 Best Companies to Work For?" list eight years in a row. Designated as a Magnet hospital for excellence in nursing. Visit us at houstonmethodist.org. Follow us at twitter.com/MethodistHosp and www.facebook.com/HoustonMethodist > > ***CONFIDENTIALITY NOTICE*** This e-mail is the property of Houston Methodist Hospital and/or its relevant affiliates and may contain restricted and privileged material for the sole use of the intended recipient(s). Any review, use, distribution or disclosure by others is strictly prohibited. If you are not the intended recipient (or authorized to receive for the recipient), please contact the sender and delete all copies of the message. Thank you. > > _______________________________________________ > Bioconductor mailing list > Bioconductor at r-project.org > https://stat.ethz.ch/mailman/listinfo/bioconductor > Search the archives: http://news.gmane.org/gmane.science.biology.informatics.conductor -- James W. MacDonald, M.S. Biostatistician University of Washington Environmental and Occupational Health Sciences 4225 Roosevelt Way NE, # 100 Seattle WA 98105-6099
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On Tue, Oct 1, 2013 at 10:56 AM, James W. MacDonald <jmacdon at="" uw.edu=""> wrote: > Hi Leif, > > Here is some code I use to do this. Here I assume you have both an > ExpressionSet called 'eset' and a PLMset called 'plm'. I usually use oligo, > so in my case it is an oligoPLM object, but no matter. > > sn <- gsub("\\.CEL", "", sampleNames(eset)) > ## don't re-make existing plots > if(!all(file.exists(paste(sn[length(sn)], "png", sep = ".")))) { > sn <- sn[!file.exists(paste(sn[length(sn)], "png", sep = "."))] > for(i in seq_len(length(sn))){ > png(paste0(sn[i], "png")) > image(plm, type = "residuals", which = i) > dev.off() > } > } FYI, with the 'R.devices' package the above can be done as: library("R.devices") ## don't re-make existing plots options("devEval/args/force"=FALSE) sn <- gsub("\\.CEL", "", sampleNames(eset)) for(i in seq_along(sn)) { toPNG(sn[i], { image(plm, type="residuals", which=i) }) } Adjust aspect ratio by specifying argument 'aspectRatio' for toPNG(). A completely different path is to use aroma.affymetrix, e.g. library("aroma.affymetrix") csR <- AffymetrixCelSet$byName("GSE9890", chipType="HG-U133_Plus_2") res <- doRMA(csR, drop=FALSE, verbose=TRUE) rs <- calculateResidualSet(res$plm, verbose=TRUE) ae <- ArrayExplorer(rs) setColorMaps(ae, c("log2,log2neg,rainbow", "log2,log2pos,rainbow")) process(ae, interleaved="auto", verbose=TRUE) display(ae) That will generate PNGs where each probe on the array occupies exactly one pixel. These PNGs can be navigated via the ArrayExplorer. See the residual ArrayExplorer example on http://aroma-project.org/demos/ for an example. /Henrik > > > Best, > > Jim > > > > On Tuesday, October 01, 2013 1:43:37 PM, Peterson, Leif wrote: >> >> We are interested in saving all the AffyPLM residual plots to disk (in a >> single folder) but cannot find syntax in the user's guide or in forums on >> how to do this. Thx ahead of time, LP >> >> Houston Methodist. Leading Medicine. >> >> Ranked by U.S.News & World Report as one of America's "Best Hospitals" in >> 13 specialties. Named to FORTUNE? Magazine's "100 Best Companies to Work >> For?" list eight years in a row. Designated as a Magnet hospital for >> excellence in nursing. Visit us at houstonmethodist.org. Follow us at >> twitter.com/MethodistHosp and www.facebook.com/HoustonMethodist >> >> ***CONFIDENTIALITY NOTICE*** This e-mail is the property of Houston >> Methodist Hospital and/or its relevant affiliates and may contain restricted >> and privileged material for the sole use of the intended recipient(s). Any >> review, use, distribution or disclosure by others is strictly prohibited. If >> you are not the intended recipient (or authorized to receive for the >> recipient), please contact the sender and delete all copies of the message. >> Thank you. >> >> _______________________________________________ >> Bioconductor mailing list >> Bioconductor at r-project.org >> https://stat.ethz.ch/mailman/listinfo/bioconductor >> Search the archives: >> http://news.gmane.org/gmane.science.biology.informatics.conductor > > > -- > James W. MacDonald, M.S. > Biostatistician > University of Washington > Environmental and Occupational Health Sciences > 4225 Roosevelt Way NE, # 100 > Seattle WA 98105-6099 > > > _______________________________________________ > Bioconductor mailing list > Bioconductor at r-project.org > https://stat.ethz.ch/mailman/listinfo/bioconductor > Search the archives: > http://news.gmane.org/gmane.science.biology.informatics.conductor
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