Dear R helpers, I'm very new to Agilent analysis and still on my self-learning the usage of 'limma'. I would like to apologize everyone if my question is too naive to be asked. I have read 'Using of Limma'. In the topic "4.7 Image-derived Spot Quality Weights", there are examples of setting weights to genepix and spot sources provided. According to my zero experience, I do have no idea how to do this properly with files of 'agilent' source. Would you please recommend me some 'should be done' settings? I really need suggestions from experienced people at this step. Best Regards, Kaj Chokeshaiusaha -- output of sessionInfo(): R version 2.15.3 (2013-03-01) Platform: x86_64-w64-mingw32/x64 (64-bit) locale: [1] LC_COLLATE=Thai_Thailand.874 LC_CTYPE=Thai_Thailand.874 [3] LC_MONETARY=Thai_Thailand.874 LC_NUMERIC=C [5] LC_TIME=Thai_Thailand.874 attached base packages: [1] stats graphics grDevices utils datasets methods base -- Sent via the guest posting facility at bioconductor.org.