Hi Anna- I've shared the Dropbox location containing all the reads for the vignette with you. I do want to ask what version of DiffBind you are using (perhaps send a sessionInfo() output?). The latest version (Bioconductor 2.13/DiffBind 1.8) has substantially new code for dba.count (using much less memory) - you may want to check this out. Also, if memory is an issue in an older version, you can set the bLowMem option to TRUE in dba.count. Cheers- Rory From: arusso <anna.russo@ieo.eu<mailto:anna.russo@ieo.eu>> Date: Fri, 18 Oct 2013 14:47:12 +0200 To: Rory Stark <rory.stark@cancer.org.uk<mailto:rory.stark@cancer.org.uk>> Subject: Problems with DiffBind Resent-From: Rory Stark <rory.stark@cancer.org.uk<mailto:rory.stark@cancer.org.uk>> Dear Dr Stark, I'm a phd student at the IEO in Milan and I'm trying to use DiffBind package but I'm having some issues using the command dba.count. I receive the segfault error that is also reported here http://grokbase.com/t/r/bioconductor/135ghztseq/bioc-diffbind-error- dba-count I used the options you describe in your answer but the error still comes up. So I wanted to ask you: 1. Can you suggest me other possible way to solve this problem? 2. Is it possible to have access to the bed files of tamoxifen dataset? In this way I can run the steps on your dataset and try to understand if there are problems with my dataset or not. Thank you very much. Kind regards, Anna ***Please note that my email address has changed. Please update your records and address book*** Anna Russo PhD student IFOM-IEO Campus via Adamello 16 20139 Milan (ITALY) Email: anna.russo@ieo.eu<mailto:anna.russo@ieo.eu> http://www.ifom-ieo-campus.it NOTICE AND DISCLAIMER This e-mail (including any attachments) is intended for ...{{dropped:20}}