Entering edit mode
Sindre
▴
110
@sindre-6193
Last seen 5.4 years ago
Thank you so much.
If genes whose response differs in diabetic patients vs healthy = 0.
Would it make any sense to "merge" the groups, thus only diabetics vs
healthy
or time point 1 vs time
point 2, to find differences there?
And also, just to be sure, is this correct?
d <- (calcNormFactors(d,method="RLE"));
d <- estimateGLMTrendedDisp(d, design, verbose=TRUE)
d <- estimateGLMTagwiseDisp(d, design)
fit <- glmFit(d,design)
Should I use other than default degrees of freedom (I have 48
samples)?
