Dear all, I'm having trouble reading FCS files created with BD FACSCalibur, CellQuest 5.2.1. The files can be opened with FlowJo and after exporting from FlowJo into FCS2 they can also be opened in R. However, I'd like to be able to load all of them with flowCore at once. Are there any known issues with this? Is there a workaround how to read them directly with flowCore? Best wishes Anja sessionInfo() R version 3.0.2 (2013-09-25) Platform: x86_64-w64-mingw32/x64 (64-bit) locale: [1] LC_COLLATE=German_Germany.1252 LC_CTYPE=German_Germany.1252 LC_MONETARY=German_Germany.1252 [4] LC_NUMERIC=C LC_TIME=German_Germany.1252 attached base packages: [1] stats graphics grDevices utils datasets methods base other attached packages: [1] flowCore_1.28.0 BiocInstaller_1.12.0 ProjectTemplate_0.4-2 testthat_0.7.1 loaded via a namespace (and not attached): [1] Biobase_2.22.0 BiocGenerics_0.8.0 evaluate_0.5.1 feature_1.2.9 graph_1.40.0 [6] ks_1.8.13 MASS_7.3-29 mvtnorm_0.9-9996 parallel_3.0.2 pcaPP_1.9-49 [11] robustbase_0.9-10 rrcov_1.3-4 stats4_3.0.2 stringr_0.6.2 tools_3.0.2 -- Dr. Anja Mirenska Team Chipcytometry Dept. Paed. Pneumology, Allergology and Neonatology I10 E02 R7370 Hannover Medical School Carl-Neuberg-Strasse 1 30625 Hannover GERMANY phone: +49-511-5327826 web: www.chipcytometry.com [[alternative HTML version deleted]]