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Last seen 10.5 years ago
I have a question about Paired analysis in EdgeR. I have read the
Users Guide (Aug 2013) and this clearly describes several types of
paired analysis and how to build the appropriate design matrixes.
However, the design closest to my experiment (4.2, p40) doesn't seem
to be a paired analysis.
My experiment is as follows: "cases" - placentas from patients with a
well defined poor obstetric outcome, "controls" placentas from 20
subjects with good obstetric outcome. The controls were selected from
a large cohort (~4000) to be a close match to the cases. Matching was
based on ~20 characteristics (maternal age, BMI, gestational age,
fetal sex, mode of delivery etc etc). Hence there is 1 to 1 matching
of cases and controls and hence a paired design. We collected RNA-Seq
data from all 40 - individually bar-codded, pooled and run on 3 lanes
of a HiSeq2000.
I am probably being a bit slow, but advice on what the design matrix
should look like would be a real help.
Thanks.
Steve
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