readVcf fails when ALT field contains breakends and no symbolic allele
1
0
Entering edit mode
@valerie-obenchain-4275
Last seen 2.9 years ago
United States
Hi Daniel, I can't reproduce this error. It looks like your version of R and Bioconductor packages are out of date. The current R version is 3.0.2 and VariantAnnotation is 1.8.2 (release) and 1.9.7 (devel). Help for updating is here, http://www.bioconductor.org/install/ Let me know if you get the same error after updating and send a small sample file if possible. Thanks. Valerie On 10/24/2013 08:58 PM, Daniel Cameron [guest] wrote: > readVcf fails to load VCF files where alternate alleles containing breakends exist but no symbolic alleles. If any variant in the VCF contains a symbolic alleles, then the VCF does load correctly (including any breakend alleles - see example below). > > > Reproduction steps: >> library(VariantAnnotation) >> readVcf("fail.vcf", "fake.fa") > Error in .Call2("new_XString_from_CHARACTER", classname, x, start(solved_SEW), : > key 91 (char '[') not in lookup table >> readVcf("success.vcf", "fake.fa") > > fail.vcf: > ##fileformat=VCFv4.1 > ##INFO=<id=svtype,number=1,type=string,description="type of="" structural="" variant"=""> > ##ALT=<id=del,description="deletion"> > #CHROM POS ID REF ALT QUAL FILTER INFO > 1 1 ref1 G G[1:2[ . PASS SVTYPE=BND > > success.vcf: > ##fileformat=VCFv4.1 > ##INFO=<id=svtype,number=1,type=string,description="type of="" structural="" variant"=""> > ##ALT=<id=del,description="deletion"> > #CHROM POS ID REF ALT QUAL FILTER INFO > 1 1 ref1 G G[1:2[ . PASS SVTYPE=BND > 1 2 ref2 G . PASS SVTYPE=DEL > > > -- output of sessionInfo(): > > R version 2.15.2 (2012-10-26) > Platform: x86_64-w64-mingw32/x64 (64-bit) > > locale: > [1] LC_COLLATE=English_Australia.1252 LC_CTYPE=English_Australia.1252 LC_MONETARY=English_Australia.1252 > [4] LC_NUMERIC=C LC_TIME=English_Australia.1252 > > attached base packages: > [1] stats graphics grDevices utils datasets methods base > > other attached packages: > [1] VariantAnnotation_1.4.12 Rsamtools_1.10.2 Biostrings_2.26.3 GenomicRanges_1.10.7 IRanges_1.16.6 > [6] BiocGenerics_0.4.0 > > loaded via a namespace (and not attached): > [1] AnnotationDbi_1.20.7 Biobase_2.18.0 biomaRt_2.14.0 bitops_1.0-6 BSgenome_1.26.1 > [6] DBI_0.2-7 GenomicFeatures_1.10.2 parallel_2.15.2 RCurl_1.95-4.1 RSQLite_0.11.4 > [11] rtracklayer_1.18.2 stats4_2.15.2 tools_2.15.2 XML_3.98-1.1 zlibbioc_1.4.0 > > -- > Sent via the guest posting facility at bioconductor.org. >
VariantAnnotation VariantAnnotation VariantAnnotation VariantAnnotation • 1.2k views
ADD COMMENT
0
Entering edit mode
@daniel-cameron-6227
Last seen 8.7 years ago
Australia
Works with R 3.0.2 / VariationAnnotation 1.8.2 for both minimal test case and my actual data. Sincere apologies for the inconvenience caused. Regards Daniel Cameron On 6/11/2013 11:25 AM, Valerie Obenchain wrote: > Hi Daniel, > > I can't reproduce this error. > > It looks like your version of R and Bioconductor packages are out of > date. The current R version is 3.0.2 and VariantAnnotation is 1.8.2 > (release) and 1.9.7 (devel). Help for updating is here, > > http://www.bioconductor.org/install/ > > Let me know if you get the same error after updating and send a small > sample file if possible. > > Thanks. > Valerie > > > On 10/24/2013 08:58 PM, Daniel Cameron [guest] wrote: >> readVcf fails to load VCF files where alternate alleles containing >> breakends exist but no symbolic alleles. If any variant in the VCF >> contains a symbolic alleles, then the VCF does load correctly >> (including any breakend alleles - see example below). >> >> >> Reproduction steps: >>> library(VariantAnnotation) >>> readVcf("fail.vcf", "fake.fa") >> Error in .Call2("new_XString_from_CHARACTER", classname, x, >> start(solved_SEW), : >> key 91 (char '[') not in lookup table >>> readVcf("success.vcf", "fake.fa") >> >> fail.vcf: >> ##fileformat=VCFv4.1 >> ##INFO=<id=svtype,number=1,type=string,description="type of="">> structural variant"> >> ##ALT=<id=del,description="deletion"> >> #CHROM POS ID REF ALT QUAL FILTER INFO >> 1 1 ref1 G G[1:2[ . PASS SVTYPE=BND >> >> success.vcf: >> ##fileformat=VCFv4.1 >> ##INFO=<id=svtype,number=1,type=string,description="type of="">> structural variant"> >> ##ALT=<id=del,description="deletion"> >> #CHROM POS ID REF ALT QUAL FILTER INFO >> 1 1 ref1 G G[1:2[ . PASS SVTYPE=BND >> 1 2 ref2 G . PASS SVTYPE=DEL >> >> >> -- output of sessionInfo(): >> >> R version 2.15.2 (2012-10-26) >> Platform: x86_64-w64-mingw32/x64 (64-bit) >> >> locale: >> [1] LC_COLLATE=English_Australia.1252 LC_CTYPE=English_Australia.1252 >> LC_MONETARY=English_Australia.1252 >> [4] LC_NUMERIC=C LC_TIME=English_Australia.1252 >> >> attached base packages: >> [1] stats graphics grDevices utils datasets methods base >> >> other attached packages: >> [1] VariantAnnotation_1.4.12 Rsamtools_1.10.2 >> Biostrings_2.26.3 GenomicRanges_1.10.7 IRanges_1.16.6 >> [6] BiocGenerics_0.4.0 >> >> loaded via a namespace (and not attached): >> [1] AnnotationDbi_1.20.7 Biobase_2.18.0 biomaRt_2.14.0 >> bitops_1.0-6 BSgenome_1.26.1 >> [6] DBI_0.2-7 GenomicFeatures_1.10.2 >> parallel_2.15.2 RCurl_1.95-4.1 RSQLite_0.11.4 >> [11] rtracklayer_1.18.2 stats4_2.15.2 tools_2.15.2 >> XML_3.98-1.1 zlibbioc_1.4.0 >> >> -- >> Sent via the guest posting facility at bioconductor.org. >> > ______________________________________________________________________ The information in this email is confidential and intend...{{dropped:4}}
ADD COMMENT
0
Entering edit mode
No problem. Glad it works for you. Valerie On 11/05/13 20:31, Daniel Cameron wrote: > Works with R 3.0.2 / VariationAnnotation 1.8.2 for both minimal test > case and my actual data. Sincere apologies for the inconvenience caused. > > Regards > Daniel Cameron > > On 6/11/2013 11:25 AM, Valerie Obenchain wrote: >> Hi Daniel, >> >> I can't reproduce this error. >> >> It looks like your version of R and Bioconductor packages are out of >> date. The current R version is 3.0.2 and VariantAnnotation is 1.8.2 >> (release) and 1.9.7 (devel). Help for updating is here, >> >> http://www.bioconductor.org/install/ >> >> Let me know if you get the same error after updating and send a small >> sample file if possible. >> >> Thanks. >> Valerie >> >> >> On 10/24/2013 08:58 PM, Daniel Cameron [guest] wrote: >>> readVcf fails to load VCF files where alternate alleles containing >>> breakends exist but no symbolic alleles. If any variant in the VCF >>> contains a symbolic alleles, then the VCF does load correctly >>> (including any breakend alleles - see example below). >>> >>> >>> Reproduction steps: >>>> library(VariantAnnotation) >>>> readVcf("fail.vcf", "fake.fa") >>> Error in .Call2("new_XString_from_CHARACTER", classname, x, >>> start(solved_SEW), : >>> key 91 (char '[') not in lookup table >>>> readVcf("success.vcf", "fake.fa") >>> >>> fail.vcf: >>> ##fileformat=VCFv4.1 >>> ##INFO=<id=svtype,number=1,type=string,description="type of="">>> structural variant"> >>> ##ALT=<id=del,description="deletion"> >>> #CHROM POS ID REF ALT QUAL FILTER INFO >>> 1 1 ref1 G G[1:2[ . PASS SVTYPE=BND >>> >>> success.vcf: >>> ##fileformat=VCFv4.1 >>> ##INFO=<id=svtype,number=1,type=string,description="type of="">>> structural variant"> >>> ##ALT=<id=del,description="deletion"> >>> #CHROM POS ID REF ALT QUAL FILTER INFO >>> 1 1 ref1 G G[1:2[ . PASS SVTYPE=BND >>> 1 2 ref2 G . PASS SVTYPE=DEL >>> >>> >>> -- output of sessionInfo(): >>> >>> R version 2.15.2 (2012-10-26) >>> Platform: x86_64-w64-mingw32/x64 (64-bit) >>> >>> locale: >>> [1] LC_COLLATE=English_Australia.1252 LC_CTYPE=English_Australia.1252 >>> LC_MONETARY=English_Australia.1252 >>> [4] LC_NUMERIC=C LC_TIME=English_Australia.1252 >>> >>> attached base packages: >>> [1] stats graphics grDevices utils datasets methods base >>> >>> other attached packages: >>> [1] VariantAnnotation_1.4.12 Rsamtools_1.10.2 >>> Biostrings_2.26.3 GenomicRanges_1.10.7 IRanges_1.16.6 >>> [6] BiocGenerics_0.4.0 >>> >>> loaded via a namespace (and not attached): >>> [1] AnnotationDbi_1.20.7 Biobase_2.18.0 biomaRt_2.14.0 >>> bitops_1.0-6 BSgenome_1.26.1 >>> [6] DBI_0.2-7 GenomicFeatures_1.10.2 >>> parallel_2.15.2 RCurl_1.95-4.1 RSQLite_0.11.4 >>> [11] rtracklayer_1.18.2 stats4_2.15.2 tools_2.15.2 >>> XML_3.98-1.1 zlibbioc_1.4.0 >>> >>> -- >>> Sent via the guest posting facility at bioconductor.org. >>> >> > > > ______________________________________________________________________ > The information in this email is confidential and inte...{{dropped:6}}
ADD REPLY

Login before adding your answer.

Traffic: 992 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6