Hi, I am currently processing Infinium HumanMethylation450K BeadChips. While looking for all the necessary steps I read the paper 'A comprehensive overview of Infinium HumanMeththylation450 data processing' by Dedeurwaerder et al. There they say that one should exclude high intensity probes from the analysis as they might cause problems. Unfortunately, I could not really get how they define 'high intensity' - whether there is a certain cut-off in terms of a percentile or an actual value. Did anyone exclude these probes during preprocessing yet and could give a hint how to determine these 'high intensity probes' or tell me whether it is useful? Thank, kind regards, Aileen