Hi there and happy new year, I was using ComBat to adjust batch effect, and it kept giving me error messages like this: Error in density.default(gamma.hat[1, ]) : 'x' contains missing values. I googled and removed rows with zero variance as suggested, but the error kept coming out. Though I ran it successfully after adding the parameter "par.prior=F", it gave an adjusted result full of "NA"s. Best, Sujun -------------- next part -------------- An embedded and charset-unspecified text was scrubbed... Name: batch_info.txt URL: <https: stat.ethz.ch="" pipermail="" bioconductor="" attachments="" 20140101="" d30b77d2="" attachment.txt=""> -------------- next part -------------- An embedded and charset-unspecified text was scrubbed... Name: dimmer_exp.txt URL: <https: stat.ethz.ch="" pipermail="" bioconductor="" attachments="" 20140101="" d30b77d2="" attachment-0001.txt="">