Currently, Pathview does not provide a single function for your particular analysis. But you can make use of the individual module functions in Pathview package to get your job done. First, make sure you update your pathview to the latest version (1.2.3). Below is an example based on what you described: multiple columns, 1 color for each column. You may also specify colors for individual nodes directly this way. Note that we do not plot the color key for such data at this time. HTH. library(pathview) #simulate data first, gene hits are 1's, others are NA's gnames <- sim.mol.data(nmol = 10000, discrete=T) gdata=matrix(NA, ncol=4, nrow=10000) rownames(gdata)=gnames idx1=sample(1:10000, 4000) idx2=sample(1:10000, 4000) idx3=sample(1:10000, 4000) idx4=sample(1:10000, 4000) gdata[idx1,1]=1 gdata[idx2,2]=1 gdata[idx3,3]=1 gdata[idx4,4]=1 #donwload, parse and map your data download.keggpathway.id="04110") xml.file="hsa04110.xml" node.data=node.info(xml.file) names(node.data) plot.data.gene=node.map(mol.data=gdata, node.data, node.types="gene") head(plot.data.gene) #generate and customize node colors cols.ts.gene=node.color(plot.data.gene, limit=2, bins=4) head(cols.ts.gene) cols=rainbow(4) bg.col="#FFFFFF" for(i in 1:4){ cols.ts.gene[cols.ts.gene[,i]!=bg.col,i]=cols[i] } #KEGG view pv.pars= keggview.native(plot.data.gene=plot.data.gene, cols.ts.gene=cols.ts.gene, node.data=node.data, pathway.name="hsa04110", same.layer=T, plot.col.key=F, out.suffix = "custom.4cols") -------------------------------------------- On Tue, 2/25/14, Simran Azariah wrote: Hi Luo, I want to map some TF enrichment data for each gene in a KEGG pathway. I am able to compartmentalize the gene boxes by using the multi.state parameter. I have been trying to pass a discrete set of colors for each representation. Is there a way we could parse a color in mol.col column for each corresponding? mol.data column. I want to show multiple TF for each gene in different colors on the KEGG pathway. I?ll be extremely grateful if you could guide me on this regards, Solomon Azariah Programmer/Analyst Geschwind lab Department of Neurology David Geffen School of Medicine UCLA