Hello, Can you please explain what the data should be taken using Differential expression using LIMMA Package. -- Kind Regards Swathi Mutyam B BCIL-BIITP Sandor Life Sciences Pvt, Ltd,. Hyderabad [[alternative HTML version deleted]]

Hi, On Mon, Mar 31, 2014 at 10:49 PM, swathi mutyam <swathimutyam91 at="" gmail.com=""> wrote: > Hello, > Can you please explain what the data should be taken using > Differential expression using LIMMA Package. You will have to provide a more detailed question if you want a reasonable answer. limma was originally designed to assess differential gene expression from microarray experiments. If you read through the limma user's guide, you should get a pretty good idea of the types of data required: http://www.bioconductor.org/packages/release/bioc/vignettes/limma/inst /doc/usersguide.pdf The limma::voom function also allows you to perform differential gene expression analysis from sequencing data. If reading this plus reading through the user's guide that I linked to above doesn't answer your question, please be more specific in your follow up correspondences on this list, as it's not quite clear what you are asking. HTH, -steve -- Steve Lianoglou Computational Biologist Genentech

Hi, Please keep all correspondence on list so that others can help (as well as learn). Use "Reply All" when replying to mails from this list. On 1 Apr 2014, at 0:43, swathi mutyam wrote: > Can you please provide some example regarding the WGDASL Differential > expression using R with only one channel(Green) I have no experience with these arrays, perhaps others here can help. However, if you search the mailing list archives for "DASL" you will get some hits: http://search.gmane.org/?query=dasl&author=&group=gmane.science.biolog y.informatics.conductor&sort=date&DEFAULTOP=and&xP=Zdasl&xFILTERS=Gsci ence.biology.informatics.conductor---A Which will likely be of interest. HTH, -steve