Extracting normalized intensities
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Gene Cutler ▴ 50
@gene-cutler-916
Last seen 10.2 years ago
I'm a little unclear on how to extract normalized intensities from a marrayNorm object. The intensities in maA appear to be the original un-normalized intensities. There are normalization factors in maMloc and maMscale, but I don't know how to apply them. The maMscale look like multiplicative values while the maMloc look additive. Thanks. Gene Cutler Research Investigator Bioinformatics Amgen SF
Normalization Normalization • 861 views
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@jean-yee-hwa-yang-104
Last seen 10.2 years ago
Hi Gene, maM extrat the normalized log-ratios. If you would like to obtain normalized single channel log-intensities. You can simply solve the equations for R and G. M = (R - G) A = (R + G) / 2 Jean On Fri, 24 Sep 2004, Gene Cutler wrote: > I'm a little unclear on how to extract normalized intensities from a > marrayNorm object. The intensities in maA appear to be the original > un-normalized intensities. There are normalization factors in maMloc > and maMscale, but I don't know how to apply them. The maMscale look > like multiplicative values while the maMloc look additive. > > Thanks. > > > > Gene Cutler > Research Investigator > Bioinformatics > Amgen SF > > _______________________________________________ > Bioconductor mailing list > Bioconductor@stat.math.ethz.ch > https://stat.ethz.ch/mailman/listinfo/bioconductor >
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