Whther can I use the normalization function to normalize my raw read counts and the perform the paired t-test to find the differicential expression genes? As we know paired t-test demands normal distribution.

hi,

We recommend you to use the DESeq() function in DESeq2, as this is likely more powerful than a paired t-test. To account for the pairs you should use a design of the form: ~ individual + condition

See also this recent thread discussing this question:

RNA-seq differentially expressed gene finding methods