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I am trying to extract information about the nucleotide signal strength at each location for a .ab1 file. Basically I want the ratio of the parameters P1AM and P2AM
library(sangerseqR) x=read.abif(file.choose()) x$P1AM
This gives me an error saying $ is not an operator for Class S4. Is there a way to achieve what I am trying to do ?
I am getting output NULL. What could be wrong ?