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SAURIN ★ 1.1k
@saurin-799
Last seen 9.7 years ago
Hi, I am getting very clear blocks of high and low and medium expression of genes across the heatmap . I am using : heatmap(esetMatrix) and using all default values in them. ------| high | low ------|-------| low | high | | (med) | |-------| Is something wrong I am doing here or heatmaps are supposed to be high low high or low high low Could anyone explain to me..if possible...please! thank you, Saurin
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@sean-davis-490
Last seen 4 months ago
United States
Saurin, What a heatmap is going to show of course depends on what expression you give it. So, a heatmap will not have any general form. If you have two groups of samples and two groups of genes, there will be four blocks, etc., but having a "medium" group does not mean that the heatmap is wrong. What structure a heatmap has depends on what is being plotted in the heatmap, not the heatmap settings (for the most part--scaling in particular can drastically change the way a heatmap looks, but the structure remains). Sean On Oct 19, 2004, at 10:15 AM, Saurin Jani wrote: > Hi, > > I am getting very clear blocks of high and low and > medium expression of genes across the heatmap . > > I am using : > > heatmap(esetMatrix) and using all default values in > them. > > ------| > high | low > ------|-------| > low | high | > | (med) | > |-------| > > > Is something wrong I am doing here or heatmaps are > supposed to be > > high low high or low high low > > > Could anyone explain to me..if possible...please! > > thank you, > Saurin > > _______________________________________________ > Bioconductor mailing list > Bioconductor@stat.math.ethz.ch > https://stat.ethz.ch/mailman/listinfo/bioconductor
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Thank you, Sean and Liu. Saurin --- Sean Davis <sdavis2@mail.nih.gov> wrote: > Saurin, > > What a heatmap is going to show of course depends on > what expression > you give it. So, a heatmap will not have any > general form. If you > have two groups of samples and two groups of genes, > there will be four > blocks, etc., but having a "medium" group does not > mean that the > heatmap is wrong. What structure a heatmap has > depends on what is > being plotted in the heatmap, not the heatmap > settings (for the most > part--scaling in particular can drastically change > the way a heatmap > looks, but the structure remains). > > Sean > > On Oct 19, 2004, at 10:15 AM, Saurin Jani wrote: > > > Hi, > > > > I am getting very clear blocks of high and low and > > medium expression of genes across the heatmap . > > > > I am using : > > > > heatmap(esetMatrix) and using all default values > in > > them. > > > > ------| > > high | low > > ------|-------| > > low | high | > > | (med) | > > |-------| > > > > > > Is something wrong I am doing here or heatmaps are > > supposed to be > > > > high low high or low high low > > > > > > Could anyone explain to me..if possible...please! > > > > thank you, > > Saurin > > > > _______________________________________________ > > Bioconductor mailing list > > Bioconductor@stat.math.ethz.ch > > https://stat.ethz.ch/mailman/listinfo/bioconductor > >
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