> Date: Mon, 24 Jan 2005 18:30:13 -0500 > From: Pita <pwilkinson_m@xbioinformatics.org> > Subject: [BioC] Using limma with contrast matrix ,replicate spots, > To: bioconductor <bioconductor@stat.math.ethz.ch> > > What is the significance of this error? What does this tell me about these > two samples?. I added the coeficients for the donor then re- calculated the fit. > > > > donor.fit = lmFit(MA, design.III) > Coefficients not estimable: HISS0039 HISS0050 This is not an error. It means just what it says: the two coefficients listed cannot be estimated. This is because the model is over-parametrized, i.e., the columns of the design matrix are not linearly independent. The model will still be fit correctly but those two coefficients will be set to NA. Gordon