dilution and spikein data
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@rafael-a-irizarry-14
Last seen 10.2 years ago
hi! in case you are interested, the Gene Logic dilution and Affymetrix spikein experiment data are available along with R friendly description files from here: http://www.biostat.jhsph.edu/~ririzarr/affycomp/ rafael
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@laurent-buffat-85
Last seen 10.2 years ago
This is a multi-part message in MIME format. ------=_NextPart_000_0002_01C2C22A.0A0C04D0 Content-Type: text/plain; charset="US-ASCII" Content-Transfer-Encoding: 7bit Hi, Thank you very much for this data. I have juste a question : why in the Affymetrix spikein experiment, you have, in the description file in your web page, 16 probes set, and in the same description file in the Affymetrix web site, only 14 probes set ? In fact, how you discovert the "33818_at" and "546_at" probes set ? Juste for information, some result of a fist analysis in the excel file. Best regards, L. Buffat -----Message d'origine----- De : bioconductor-admin@stat.math.ethz.ch [mailto:bioconductor-admin@stat.math.ethz.ch]De la part de Rafael A. Irizarry Envoye : jeudi 23 janvier 2003 23:47 A : bioconductor@stat.math.ethz.ch Cc : wfan@fhcrc.org Objet : [BioC] dilution and spikein data hi! in case you are interested, the Gene Logic dilution and Affymetrix spikein experiment data are available along with R friendly description files from here: http://www.biostat.jhsph.edu/~ririzarr/affycomp/ rafael _______________________________________________ Bioconductor mailing list Bioconductor@stat.math.ethz.ch http://www.stat.math.ethz.ch/mailman/listinfo/bioconductor ------=_NextPart_000_0002_01C2C22A.0A0C04D0 Content-Type: application/vnd.ms-excel; name="SpikeIn.res.xls" Content-Transfer-Encoding: base64 Content-Disposition: attachment; filename="SpikeIn.res.xls" 0M8R4KGxGuEAAAAAAAAAAAAAAAAAAAAAPgADAP7/CQAGAAAAAAAAAAAAAAABAAAALAAAAA AAAAAA EAAA/v///wAAAAD+////AAAAACsAAAD/////////////////////////////////////// ////// ////////////////////////////////////////////////////////////////////// ////// 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good question. we are almost certain that affy forgot to mention 2 genes that were spiked-in. more information will be made available through the web-page http://www.biostat.jhsph.edu/~ririzarr/affycomp.html -rafael On Wed, 22 Jan 2003, buffat wrote: > > Hi, > > Thank you very much for this data. > I have juste a question : > > why in the Affymetrix spikein experiment, you have, in the description file > in your web page, 16 probes set, and in the same description file in the > Affymetrix web site, only 14 probes set ? > In fact, how you discovert the "33818_at" and "546_at" probes set ? > > Juste for information, some result of a fist analysis in the excel file. > > Best regards, > > L. Buffat > > -----Message d'origine----- > De : bioconductor-admin@stat.math.ethz.ch > [mailto:bioconductor-admin@stat.math.ethz.ch]De la part de Rafael A. > Irizarry > Envoye : jeudi 23 janvier 2003 23:47 > A : bioconductor@stat.math.ethz.ch > Cc : wfan@fhcrc.org > Objet : [BioC] dilution and spikein data > > > hi! > > in case you are interested, the Gene Logic dilution and > Affymetrix spikein experiment data are available along > with R friendly description files from here: > > http://www.biostat.jhsph.edu/~ririzarr/affycomp/ > > rafael > > _______________________________________________ > Bioconductor mailing list > Bioconductor@stat.math.ethz.ch > http://www.stat.math.ethz.ch/mailman/listinfo/bioconductor >
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Hi, Thanks for reply so fast. I have anover question : In my analysis of these data, I have somes "false positive" what I retreive between differents conditions. For example "32660_at" is "positive" in a vs b, b vs c, c vs d and m-p vs q-t with a very good rank (8,2,2,16) Do you thinks they also "forgot to mention it" ? Thanks, Laurent -----Message d'origine----- De : Rafael A. Irizarry [mailto:ririzarr@jhsph.edu] Envoye : mercredi 29 janvier 2003 16:27 A : buffat Cc : bioconductor@stat.math.ethz.ch; wfan@fhcrc.org Objet : RE: [BioC] dilution and spikein data good question. we are almost certain that affy forgot to mention 2 genes that were spiked-in. more information will be made available through the web-page http://www.biostat.jhsph.edu/~ririzarr/affycomp.html -rafael On Wed, 22 Jan 2003, buffat wrote: > > Hi, > > Thank you very much for this data. > I have juste a question : > > why in the Affymetrix spikein experiment, you have, in the description file > in your web page, 16 probes set, and in the same description file in the > Affymetrix web site, only 14 probes set ? > In fact, how you discovert the "33818_at" and "546_at" probes set ? > > Juste for information, some result of a fist analysis in the excel file. > > Best regards, > > L. Buffat > > -----Message d'origine----- > De : bioconductor-admin@stat.math.ethz.ch > [mailto:bioconductor-admin@stat.math.ethz.ch]De la part de Rafael A. > Irizarry > Envoye : jeudi 23 janvier 2003 23:47 > A : bioconductor@stat.math.ethz.ch > Cc : wfan@fhcrc.org > Objet : [BioC] dilution and spikein data > > > hi! > > in case you are interested, the Gene Logic dilution and > Affymetrix spikein experiment data are available along > with R friendly description files from here: > > http://www.biostat.jhsph.edu/~ririzarr/affycomp/ > > rafael > > _______________________________________________ > Bioconductor mailing list > Bioconductor@stat.math.ethz.ch > http://www.stat.math.ethz.ch/mailman/listinfo/bioconductor >
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