Entering edit mode
Rafael A. Irizarry
▴
640
@rafael-a-irizarry-14
Last seen 10.3 years ago
this is what you have to do.
in the exprs (intensity) slot of an AffyBatch put a matrix with as
many
columns as arrays and as many row as number of probes on the array.
in each column put the vector of intensities of each array. a row must
represent a unique location on the array. no you need to create a hash
table enviromnet with varialbles with names of the probesets. the
variabes
need to be 2-column matrix where the first column has the positions of
the
PMs of that probe set and the second row the MMs. for example the
variable
1000_at
could be
1 641
2 642
12 652
etc..
now in the cdfName slot of the exprset put the name of this
enviromnet.
this should get you on your way. look at the example
data(Dilution)
to get a better idea. you will that the environment (loaded through a
library) hgu95av2cdf has the variables 1000_at, 1001_at, etc... with
the
positions.
hope this helps,
-r
On Tue, 4 Feb 2003, Tibor van Rooij wrote:
> Hi! I would very much like to use the MBEI method, however all my
data is stored in a database. I can easily access data such as
probename x,y positions, pm value mm value etcetera. In the previous
version of "affy" I could access slotnames of the objects such as
names to put my names into and pm to put my pm values in and get the
sytem to calculate the normalization ( so I did not need the original
.CEL files)
>
> I have been looking at the descriptions of the new classes and
reading the manual but I cannot find out how to this in this new
version.
>
> Has anybody else attempted this?
>
> Please let me know.
>
> Tibor van Rooij
> Bioinformatician
> McGill University and
> Genome Quebec Innovation Centre
> 740 Dr. Penfield Avenue, Rm 6212
> Montreal, Quebec H3A 1A4
> Tel: (514) 398-2497
>
>
> [[alternate HTML version deleted]]
>
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