lymphGate Preselection Argument
0
0
Entering edit mode
alee • 0
@alee-9968
Last seen 9.7 years ago

I attempted to use lymphGate to select the lymphocyte population from 10 FCS files.  I used the following code:

rg <- rectangleGate(filterId="preselection", list("FSC-A"=c(20, 125),"SSC-A"=c(0, 100)))

lg <- flowStats::lymphGate(tflowData, channels=c("FSC-A", "SSC-A"), , preselection = "APC-eFluor 780-A", rectDef = rg, scale = 1.0, bwFac=1.3, evaluate=TRUE)
xyplot(`SSC-A`~`FSC-A`, tflowData, filter =  lg$n2gate, layout = c(5,2))

 

Based on the lymphGate documentation, I expected that I would input the parameter that defines a rectangle ("rg") and then lymphGate ("lg") would fit a bivariate normal distribution to the cells contained within the preselected rectangle ("rg").  Then it would identify an elliptical population within the rectangle.

However, the results I got selected an ellipsoid that is outside of the rectangle.  Why is this happening with the preselection parameter?

 

 

 

 

 

lymphgate • 1.1k views
ADD COMMENT

Login before adding your answer.

Traffic: 606 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6