Hi,
I'm getting the following error:
> library("IONiseR", lib.loc="C:/Program Files/R/R-3.3.1/library")
> library("ggplot2", lib.loc="C:/Program Files/R/R-3.3.1/library")
> library("gridExtra", lib.loc="C:/Program Files/R/R-3.3.1/library")
> setwd("E:\\data\\reads\\downloads\\pass")
> getwd()
[1] "E:/data/reads/downloads/pass"
> fast5files <- list.files(path = getwd(), pattern = ".fast5$", full.names = TRUE)
> data.summary <- readFast5Summary(fast5files)
Checking file validity
Reading Channel Data
Reading Raw Data
Reading Template Data
Reading Complement Data
Reading Template FASTQ
Error in .subset(x, j) : invalid subscript type 'closure'
R 3.3.1 and tried IoniseR 1.2.2 and then 1.3.2, getting the same error. Previous version of IoniseR 1.1.x under R 3.2.x used to work...
Thanks,
Ron
Hi Ron,
The format of the fast5 files changes quite frequently, so it maybe that IONiseR is unable to cope with some change in the internal structure. Can you make one or two fast5 files available to me? (I don't need to know anything about the sample that were sequenced). It would really help me get to the bottom of what's causing the error.
If you don't want to post a link on a public site you can send an email to the address in the package DESCRIPTION file.
Cheers,
Mike