I have used the command with success many times until yesterday, I did some package updates since last success that may explain this but have no clue on what to do now.
This looks like an old issue I found but does not seem to be recently reported. All my packages have been deinstalled and reinstalled without improvement.
Using R 3.3.0 under RStudio (BSgenome v1.4.0 ; TxDb.Hsapiens.UCSC.hg19.knownGene 3.2.2; VariantAnnotation 1.18.7)
Thanks for your help
Here is a significant part of my code
library("VariantAnnotation") library("TxDb.Hsapiens.UCSC.hg19.knownGene") library("BSgenome.Hsapiens.UCSC.hg19") vcf <- readVcf(vcf_file, "hg19") Warning message: In .bcfHeaderAsSimpleList(header) : duplicate keys in header will be forced to unique rownames # but the VCF is OK and I can do many things with it except the following: # annotation database txdb <- "TxDb.Hsapiens.UCSC.hg19.knownGene" # locate coding variants pred.coding <- predictCoding(vcf, txdb, Hsapiens) Error in (function (classes, fdef, mtable) : unable to find an inherited method for function ‘predictCoding’ for signature ‘"CollapsedVCF", "character", "BSgenome", "missing"’ showMethods("predictCoding") Function: predictCoding (package VariantAnnotation) query="CollapsedVCF", subject="TxDb", seqSource="ANY", varAllele="missing" query="ExpandedVCF", subject="TxDb", seqSource="ANY", varAllele="missing" query="GRanges", subject="TxDb", seqSource="ANY", varAllele="DNAStringSet" query="Ranges", subject="TxDb", seqSource="ANY", varAllele="DNAStringSet" query="VRanges", subject="TxDb", seqSource="ANY", varAllele="missing" > sessionInfo() R version 3.3.0 (2016-05-03) Platform: x86_64-apple-darwin13.4.0 (64-bit) Running under: OS X 10.10.5 (Yosemite) locale: [1] en_US.UTF-8/en_US.UTF-8/en_US.UTF-8/C/en_US.UTF-8/en_US.UTF-8 attached base packages: [1] stats4 parallel stats graphics grDevices utils datasets methods base other attached packages: [1] BiocInstaller_1.22.3 BSgenome.Hsapiens.UCSC.hg19_1.4.0 [3] BSgenome_1.40.1 rtracklayer_1.32.2 [5] TxDb.Hsapiens.UCSC.hg19.knownGene_3.2.2 GenomicFeatures_1.24.5 [7] AnnotationDbi_1.34.4 VariantAnnotation_1.18.7 [9] Rsamtools_1.24.0 Biostrings_2.40.2 [11] XVector_0.12.1 SummarizedExperiment_1.2.3 [13] Biobase_2.32.0 GenomicRanges_1.24.3 [15] GenomeInfoDb_1.8.7 IRanges_2.6.1 [17] S4Vectors_0.10.3 BiocGenerics_0.18.0 [19] biomaRt_2.28.0 reshape2_1.4.1 [21] ggplot2_2.1.0 RColorBrewer_1.1-2 [23] pheatmap_1.0.8 knitr_1.14 loaded via a namespace (and not attached): [1] Rcpp_0.12.7 plyr_1.8.4 bitops_1.0-6 tools_3.3.0 [5] zlibbioc_1.18.0 RSQLite_1.0.0 gtable_0.2.0 DBI_0.5-1 [9] stringr_1.1.0 grid_3.3.0 XML_3.98-1.4 BiocParallel_1.6.6 [13] magrittr_1.5 scales_0.4.0 GenomicAlignments_1.8.4 colorspace_1.2-6 [17] stringi_1.1.2 RCurl_1.95-4.8 munsell_0.4.3
It does not seems to be my data as it also fails using package demo data (all packages already loaded as above)