Hi, I'm Bárbara Gutiérrez from Chile, I'm studying biochemestry in Universidad de Chile and I'm in genetic engineering course. I have to do a job with FindMyFriends Package and R studio and I need some help.
This is my theoretical strategy:
First we must collect the DNA of the microorganisms in the sample and then fragment it to sequence it. Then the known sequences are stored in a FASTA file, which will then be entered into an array (I have my dataset), where I will calculate the similarity and identity between them (x), I will consider possible spontaneous mutations so I will do an algorithm of Probability (I will manipulate Y) and finally I will make a phylogenetic analysis of these sequences (to do z) where I will obtain the phylogenetic tree (in diagram or whatever, this would answer question 8) (ABC).
And my professor ask me how is the workflow with the necessary commands in R to carry out my strategy.. and I dont know how to do this!!