Hello all, I have question about global normalization. I have data from big experement(two-color cDNA arrays) where used three different layouts but the same set of genes. I used limma package for within array normalization. My question is: how I can do between array normalization (global) if arrays have different layout(different location and different number technical replicates (number of spots of a gene on an array))? Sincerely, Natalia.