hi,I run the code successfully:
yap <- dba.count(yap)
yap <- dba.contrast(yap,categories=DBA_CONDITION,minMembers=2)
yap
4 Samples, 31676 sites in matrix:
ID Condition Treatment Replicate Caller Intervals FRiP
1 IP1 IP IP 1 counts 31676 0.53
2 IP2 IP IP 2 counts 31676 0.56
3 IgG1 IgG IgG 1 counts 31676 0.46
4 IgG2 IgG IgG 2 counts 31676 0.46
but when I uesd the'dba.analyze',it shows that:
yap <- dba.analyze(yap)
converting counts to integer mode
Error in .local(object, ...) :
all genes have equal values for all samples. will not be able to perform differential analysis
In addition: Warning message:
In DESeqDataSet(se, design = design, ignoreRank) :
all genes have equal values for all samples. will not be able to perform differential analysis
and why when i completed the 'dba.contrast', it shows the same 'Intervals'. thank you!!!
thank you, Rory! The same bam file is being used for the multiple samples.
Are you trying to cheat your way around a lack of replicates?
yes,I am trying other methods.
If you have no replicates, no serious software will work. Lying to the software you are using yields garbage.
The seq file I used was downloading from GEO,unfortunately there is not replicates!
sorry, I have another trouble.
And when I use the data that last time I used also return this erro. Thank you !