Here is the latter part of the code but towards the end an error message pops up that teminates the instruction - any suggestions appreciated.....(I only have a basic grasp of R)

```> # The argument second_fractionFR must be greater or equal to timestep.

fcstimestep <- as.numeric(inputFCS@description[['$TIMESTEP']]) if ((length(fcstimestep) > 0) && fj_second_fractionFR < fcstimestep) fj_second_fractionFR <- fcstimestep

newFlowFrame <- flow_auto_qc(

• inputFCS,
• output = 2,
• ChExcludeFS = ChRemoveFS,
• remove_from = "all",
• second_fractionFR = fj_second_fractionFR,
• max_cptFS = 3.0,
• pen_valueFS = pen_valueFS,
• outlier_binsFS = FALSE,
• decompFR = TRUE,
• sideFM = "both",
• neg_valuesFM = 1,
• fcs_QC = FALSE,
• folder_results = FALSE,
• html_report = TRUE, ## We extract the figures from this
• alphaFR = 0.01) Quality control for the file: 180313A PBMC CD127 BV421 Titration_180313A CD127 Titration_Tube 2 4ul 5.24% of anomalous cells detected in the flow rate check. 16.2% of anomalous cells detected in signal acquisition check. 5.68% of anomalous cells detected in the dynamic range check. Error in file(con, "w") : cannot open the connection Calls: flow_auto_qc -> knit2html -> <Anonymous> -> writeLines -> file In addition: Warning messages: 1: In file.create(to[okay]) : cannot create file '180313A PBMC CD127 BV421 Titration_180313A CD127 Titration_Tube 2 4ul_template.Rmd', reason 'No such file or directory' 2: In file(con, "w") : cannot open file '180313A PBMC CD127 BV421 Titration_180313A CD127 Titration_Tube 2 4ulTRUE.html': No such file or directory Execution halted