Question

Error in DESeq2

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mithu18mohan • 0

@4bede5c6

Last seen 3.9 years ago

India

Hi, All I am new to Rstudio , i Have to study expression data for the following But my DESeq2 data is having an error of Error in h(simpleError(msg, call)) : error in evaluating the argument 'x' in selecting a method for function 'ncol': object 'countData' not found

It would be helpful if you could provide with solution Thanks in Advance


dds <- DESeqDataSetFromMatrix(countData=countData,colData=metaData,design=~dex, tidy = TRUE)

sessionInfo( )

DESeq2 • 3.0k views

ADD COMMENT • link updated 3.9 years ago by Michael Love 43k • written 3.9 years ago by mithu18mohan • 0

score 0 · Answer 1 · 2021-12-06

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Basti ▴ 780

@7d45153c

Last seen 22 days ago

France

object 'countData' not found so you don't have any countData object loaded, it should be a matrix of counts created before running this code

ADD COMMENT • link 3.9 years ago Basti ▴ 780

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Thanks Basti for reply ,

CountData <- read.csv('om_060_12.csv', header = TRUE, sep = ",")

I have added the count data and the headcount gives the result. still, The error is not solved.

ADD REPLY • link 3.9 years ago mithu18mohan • 0

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CountData is different from countData, please check the names of the object you are calling in DESeqDataSetFromMatrix

ADD REPLY • link 3.9 years ago Basti ▴ 780