Understanding the output from dasper::junction_annotation()
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zli16 • 0
@2528c6c5
Last seen 20 months ago
United States

Dear dasper developer:

I was wondering how to interprete the output from the junction_annotation() function. For example, my dataset was annotated to the following labels:

annotated novel_acceptor novel_donor novel_exon_skip novel_combo ambig_gene unannotated

I wonder how to understand these labels. In the function manual, it worte: junction_annot annotates junctions by 1. whether their start and/or end position precisely overlaps with an annotated exon boundary and 2. whether that junction matches an intron definition from existing annotation. Using this information along with the strand, junctions are categorised into "annotated", "novel_acceptor", "novel_donor", "novel_combo", "novel_exon_skip", "ambig_gene" and "unannotated"

However, it is still unclear what "novel_acceptor", "novel_donor", "novel_combo", "ambig_gene" mean. Very much appreciate if you can direct me to any related references.

dasper • 531 views
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