hello everyone i am trying to prform differntial gene expression using the ima package u i am receiving the following error. the due date for the assignment is very close I will be glad if anyone could help

+     makeContrasts("Heart_Diabetic_failure-Normal",
+                   "Heart_Non_Diabetic_failure-Normal",
+                   levels=design);
> fit2 <- contrasts.fit(fit, contrast.matrix)
> fit2 <- eBayes(fit2)
Error in .ebayes(fit = fit, proportion = proportion, stdev.coef.lim = stdev.coef.lim,  : 
  No residual degrees of freedom in linear model fits

See previous answer to the same question: ERROR: No residual degrees of freedom in linear model fits