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Hi everyone,
I need help please. I keep getting this error in the WGCNA What could I be doing wrong?
Thanks in advance
[1] 20 3
> dim(moduleTraitCor)
[1] 401 3
> class(design2)
[1] "matrix" "array"
> class(moduleTraitCor)
[1] "matrix" "array"
> # Display the correlation values within a heatmap plot
> labeledHeatmap(Matrix = moduleTraitCor,
+ xLabels = names(design2),
+ yLabels = names(MEs),
+ ySymbols = names(MEs),
+ colorLabels = FALSE,
+ colors = greenWhiteRed(50),
+ textMatrix = textMatrix,
+ setStdMargins = FALSE,
+ cex.text = 0.5,
+ zlim = c(-1,1),
+ main = paste("Module-trait relationships"))
Error in labeledHeatmap(Matrix = moduleTraitCor, xLabels = names(design2), :
Length of 'xLabels' must equal the number of columns in 'Matrix.'
sessionInfo( )
R version 4.0.4 (2021-02-15)
Platform: x86_64-w64-mingw32/x64 (64-bit)
Running under: Windows 10 x64 (build 19044)
Matrix products: default
locale:
[1] LC_COLLATE=English_United States.1252 LC_CTYPE=English_United States.1252
[3] LC_MONETARY=English_United States.1252 LC_NUMERIC=C
[5] LC_TIME=English_United States.1252
attached base packages:
[1] parallel stats4 stats graphics grDevices utils datasets methods base
other attached packages:
[1] flashClust_1.01-2 WGCNA_1.70-3 fastcluster_1.2.3 dynamicTreeCut_1.63-1
[5] edgeR_3.32.1 limma_3.46.0 DESeq2_1.30.1 SummarizedExperiment_1.20.0
[9] Biobase_2.50.0 MatrixGenerics_1.2.1 matrixStats_0.61.0 GenomicRanges_1.42.0
[13] GenomeInfoDb_1.26.7 IRanges_2.24.1 S4Vectors_0.28.1 BiocGenerics_0.36.1
loaded via a namespace (and not attached):
[1] colorspace_2.0-2 ellipsis_0.3.2 class_7.3-19 htmlTable_2.3.0
[5] XVector_0.30.0 base64enc_0.1-3 rstudioapi_0.13 listenv_0.8.0
[9] bit64_4.0.5 AnnotationDbi_1.52.0 prodlim_2019.11.13 fansi_0.5.0
[13] lubridate_1.8.0 codetools_0.2-18 splines_4.0.4 doParallel_1.0.16
[17] impute_1.64.0 cachem_1.0.6 knitr_1.36 geneplotter_1.68.0
[21] Formula_1.2-4 pROC_1.18.0 caret_6.0-90 annotate_1.68.0
[25] cluster_2.1.2 GO.db_3.12.1 png_0.1-7 pheatmap_1.0.12
[29] BiocManager_1.30.16 compiler_4.0.4 httr_1.4.2 backports_1.3.0
[33] assertthat_0.2.1 Matrix_1.3-4 fastmap_1.1.0 htmltools_0.5.2
[37] tools_4.0.4 gtable_0.3.0 glue_1.4.2 GenomeInfoDbData_1.2.4
[41] reshape2_1.4.4 dplyr_1.0.7 Rcpp_1.0.7 vctrs_0.3.8
[45] preprocessCore_1.52.1 nlme_3.1-153 iterators_1.0.13 timeDate_3043.102
[49] xfun_0.28 gower_0.2.2 stringr_1.4.0 globals_0.14.0
[53] lifecycle_1.0.1 XML_3.99-0.8 future_1.23.0 zlibbioc_1.36.0
[57] MASS_7.3-54 scales_1.1.1 ipred_0.9-12 RColorBrewer_1.1-2
[61] memoise_2.0.0 gridExtra_2.3 ggplot2_3.3.5 rpart_4.1-15
[65] latticeExtra_0.6-29 stringi_1.7.5 RSQLite_2.2.8 genefilter_1.72.1
[69] foreach_1.5.1 checkmate_2.0.0 BiocParallel_1.24.1 lava_1.6.10
[73] rlang_0.4.12 pkgconfig_2.0.3 bitops_1.0-7 lattice_0.20-45
[77] purrr_0.3.4 htmlwidgets_1.5.4 recipes_0.1.17 bit_4.0.4
[81] tidyselect_1.1.1 parallelly_1.28.1 plyr_1.8.6 magrittr_2.0.1
[85] R6_2.5.1 generics_0.1.1 Hmisc_4.6-0 DelayedArray_0.16.3
[89] DBI_1.1.1 pillar_1.6.4 foreign_0.8-81 withr_2.4.2
[93] survival_3.2-13 RCurl_1.98-1.5 nnet_7.3-16 tibble_3.1.5
[97] future.apply_1.8.1 crayon_1.4.2 utf8_1.2.2 jpeg_0.1-9
[101] locfit_1.5-9.4 grid_4.0.4 data.table_1.14.2 blob_1.2.2
[105] ModelMetrics_1.2.2.2 digest_0.6.28 xtable_1.8-4 munsell_0.5.0

Length of 'xLabels' must equal the number of columns in 'Matrix.indicates that you provided more trait labels in names(design2) than how much it is present incolnames(moduleTraitCor).Thank you for your reply , The dim function shows that both have the same column number
I resolved the issue by changing design2 into a data frame.
Many thanks.