ASICS - Include internal standard in analysis

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Pere ▴ 10

@88b8445d

Last seen 23 months ago

Spain

I have run the ASICS package to analyse H-NMR spectra from urine samples. I have correctly obtained the relative concentration of multiple metabolites. However, I would like now to add the internal standard spectra in the pure spectra library in order to calculate the absolute concentration. I am using TMSP, which has a ppm = 0. If I am not wrong, the spectra analysis starts at 0.5 ppm, so I cannot obtain the data refering to TMSP peak. Is there any way to solve this issue?

Many thanks in advance!

ASICS asic • 1.3k views

ADD COMMENT • link 2.5 years ago • updated 23 months ago Pere ▴ 10

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Hi Pere, Did you ever find a solution? I am facing the same problem. Kind regards, Chris

ADD REPLY • link 23 months ago ctj.wall • 0

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Hi Chris,

Unfortunately I have not find any solution to the problem. I tried to change the ppmgrid, making it start at -0.5ppm and including the info for TMSP, but the problem is that all the other signals are also moved and therefore the analysis is incorrect. I could not think of any other solutions so, at last, I gave up on ASICS package and performed the analysis differently.

Kind regards,

Pere

ADD REPLY • link 23 months ago Pere ▴ 10

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