Entering edit mode
My collaborator is requesting the following -
A single DEG containing fold change STIM vs. UNS in MDD vs. fold change STIM vs. UNS in HC
I would like to know, it this possible with DESeq2? I am currently using the model ~ PatientID + Stimulation * Group
Following is the metadata -
| PatientID | Stimulation | Group |
|---|---|---|
| 1 | UNS | MDD |
| 1 | STIM | MDD |
| 2 | UNS | MDD |
| 2 | STIM | MDD |
| 3 | UNS | MDD |
| 3 | STIM | MDD |
| 4 | UNS | MDD |
| 4 | STIM | MDD |
| 5 | UNS | MDD |
| 5 | STIM | MDD |
| 1 | UNS | HC |
| 1 | STIM | HC |
| 2 | UNS | HC |
| 2 | STIM | HC |
| 3 | UNS | HC |
| 3 | STIM | HC |
Thank you! It took me a while to wrap my head around the concept of interaction terms in DESeq2 model.