Entering edit mode
I'm trying to use a quad gate to identify CD56++ (double positive) CD16+ cells. As such, I'm trying to force the quadrantGate function do find the separation point on the high side of the major CD56 signal peak (the CD16 separation point is perfect). Unfortunately I can only get it to identify the separation point on the low side for CD56 which is not what what I want. Grateful for any suggestions.
flowStats::quadrantGate(df, stains=c("CD16", "CD56"), plot=TRUE)
To answer my own question, using the rare=TRUE option moves the CD56 gate to the top of the distribution quite nicely.