Question

Alternatives to Voom in experiments without replicate

0

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Mohammad • 0

@faf1c3a8

Last seen 2.0 years ago

United States

I don't have replicates for two of the species I'm working on, I can't use Voom as it requires biological replicates. What is the alternative (s)?

Your opinions and comments are welcomed.

limma edgeR Voom • 754 views

ADD COMMENT • link updated 2.0 years ago by Gordon Smyth 52k • written 2.0 years ago by Mohammad • 0

score 1 · Answer 1 · 2022-12-30

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Gordon Smyth 52k

@gordon-smyth

Last seen 19 hours ago

WEHI, Melbourne, Australia

There is no analog to voom in the absence of replicates. See the section "What to do if you don't have replicates" in the edgeR User's Guide.

ADD COMMENT • link 2.0 years ago Gordon Smyth 52k