Diffbind (or maybe DESeq2) Fold clarification
1
0
Entering edit mode
slrpatty • 0
@3f073f21
Last seen 22 months ago
United States

I'm comparing knockdown ChIP (3 replicates in the green) versus control ChIP (3 replicates in the orange), but the Fold (in the grey) is much lower than I expected it to be. I know the Fold isn't just Log2FC. Could the low Fold value be caused by shrinkage adjustments due to inconsistencies between the replicates? enter image description here

DiffBind • 717 views
ADD COMMENT
0
Entering edit mode

Note the posting guide, and instructions on including relevant code.

ADD REPLY
0
Entering edit mode
Rory Stark ★ 5.2k
@rory-stark-5741
Last seen 5 weeks ago
Cambridge, UK

The fold changes in the DiffBind report are indeed those calculated by DESeq2 after calling DESeq2::lfcShrink(). The help page for DESeq2::lfcShrink includes a number of citations for papers that describe these calculations in more detail.

ADD COMMENT

Login before adding your answer.

Traffic: 617 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6