Hi Rory Stark，

  DiffBind normalization based on the total reads of a bam file by default, right? And the final results of DiffBind: Conc_Resistant and Conc_Responsive which as the vignette suggested were the log2 (mean normalized reads of each group). So, the mean normalized reads were reads from each peak region of each group? Am I correct?  Hope get your answer to clarify my confusion.

Thanks in advance.

Tao

Addressed in issue DiffBind---Normalization problem-spike in or no spike in beforehand?