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Hello, I have designed some primers with openPrimeR. On the manual, the object with the primers is saved as a temporary file with tempfile(). Is it possible to save it on a local directory of my choice? Thank you
out.file <- tempfile("my_primers", fileext = ".fasta")
write_primers(optimal.primers$opti, out.file)
# Define the FASTA primer file to load <<< this load an external file, I would like to re-open the one I made... <<<<
# ----------------------------------------------------------------------
primer.location <- system.file("extdata", "IMGT_data", "primers", "IGHV",
"Ippolito2012.fasta", package = "openPrimeR")
# Load the primers
primer.df <- read_primers(primer.location, fw.id = "_fw")
Thank you
> sessionInfo( )
R version 4.0.3 (2020-10-10)
Platform: x86_64-pc-linux-gnu (64-bit)
Running under: Ubuntu 20.04.2 LTS
Matrix products: default
BLAS: /usr/lib/x86_64-linux-gnu/blas/libblas.so.3.9.0
LAPACK: /usr/lib/x86_64-linux-gnu/lapack/liblapack.so.3.9.0
locale:
[1] LC_CTYPE=en_US.UTF-8 LC_NUMERIC=C LC_TIME=en_GB.UTF-8 LC_COLLATE=en_US.UTF-8
[5] LC_MONETARY=en_GB.UTF-8 LC_MESSAGES=en_US.UTF-8 LC_PAPER=en_GB.UTF-8 LC_NAME=C
[9] LC_ADDRESS=C LC_TELEPHONE=C LC_MEASUREMENT=en_GB.UTF-8 LC_IDENTIFICATION=C
attached base packages:
[1] stats graphics grDevices utils datasets methods base
other attached packages:
[1] openPrimeR_1.12.1
loaded via a namespace (and not attached):
[1] progress_1.2.2 tidyselect_1.1.0 purrr_0.3.4 reshape2_1.4.4
[5] colorspace_2.0-0 DECIPHER_2.18.1 vctrs_0.3.6 generics_0.1.0
[9] stats4_4.0.3 blob_1.2.1 XML_3.99-0.5 rlang_0.4.10
[13] pillar_1.4.7 glue_1.4.2 DBI_1.1.1 BiocGenerics_0.36.0
[17] bit64_4.0.5 RColorBrewer_1.1-2 GenomeInfoDbData_1.2.4 foreach_1.5.1
[21] lifecycle_1.0.0 plyr_1.8.6 stringr_1.4.0 zlibbioc_1.36.0
[25] Biostrings_2.58.0 munsell_0.5.0 gtable_0.3.0 lpSolveAPI_5.5.2.0-17.7
[29] codetools_0.2-18 memoise_2.0.0 IRanges_2.24.1 fastmap_1.1.0
[33] GenomeInfoDb_1.26.2 parallel_4.0.3 Rcpp_1.0.6 scales_1.1.1
[37] cachem_1.0.4 S4Vectors_0.28.1 XVector_0.30.0 startupmsg_0.9.6
[41] bit_4.0.4 ggplot2_3.3.3 hms_1.0.0 stringi_1.5.3
[45] uniqtag_1.0 dplyr_1.0.4 distr_2.8.0 GenomicRanges_1.42.0
[49] grid_4.0.3 ade4_1.7-16 tools_4.0.3 bitops_1.0-6
[53] magrittr_2.0.1 RCurl_1.98-1.2 tibble_3.0.6 RSQLite_2.2.3
[57] crayon_1.4.1 seqinr_4.2-5 pkgconfig_2.0.3 ellipsis_0.3.1
[61] MASS_7.3-53.1 prettyunits_1.1.1 rstudioapi_0.13 iterators_1.0.13
[65] R6_2.5.0 sfsmisc_1.1-8 compiler_4.0.3
Hi, I tried to do the same but I dont think that my file was saved
what can I do?