LEfse %>% 
   ggdiffclade(obj=.,
               alpha=0.3, linewd=0.2, 
               skpointsize=0.6,
               taxlevel=3,
               settheme=FALSE, 
               setColors=FALSE,
               cladetext=1.5, removeUnknown = F) +
   guides(color = guide_legend(keywidth = 0.1,
                               keyheight = 0.6,
                               order = 3, 
                               ncol=1)) + 
   scale_fill_manual(values=c("#008B45FF","#EE0000FF","purple")) +

   guides(fill=guide_legend(order = 1,reverse = T,keyheight = 0.1,keywidth = 0.6))+
   theme(panel.background=element_rect(fill=NA),
         legend.position="right",
         plot.margin=margin(0,0,0,0),
         legend.spacing.x = unit(0.02, "cm"),
         legend.title=element_text(size=7),
         legend.text=element_text(size=6),
         legend.box.spacing=unit(0.02,"cm"))
 Invaild edge matrix for <phylo>. A <tbl_df> is returned.
 Invaild edge matrix for <phylo>. A <tbl_df> is returned.
 Invaild edge matrix for <phylo>. A <tbl_df> is returned.
 Invaild edge matrix for <phylo>. A <tbl_df> is returned.
Error in offspring.tbl_tree_item(.data = .data, .node = .node, tiponly = tiponly,  : 
  could not find function "offspring.tbl_tree_item"

See the last message here.

It seems there is an issue with the phylogenetic tree data or the custom function offspring.tbl_tree_item. To resolve the error, ensure the tree is properly formatted, and make sure the required packages for handling phylogenetic data are loaded. Additionally, Content Stop offers student academic assistance through its assignment writing service.