Hi all,

I have a questions about bulk ATAc seq that could not find out myself and also on Biostar. I use Diffbind to get the table below (differential accessibility regions) and then use ChipPeakAnno to annotate the peaks in the P column. Could I conclude that gene with positive Fold (D columns) is more open in diseased than in control (B column vs C column)? Base on the peaks on IGV, could I say this gene in diseased is more chromatin open than the control?Thank you so much!

You have probably not got a response because it looks like you are either joking or trolling. But maybe you are not, so here goes.

You appear to realize that column B is a measure of open chromatin in the diseased samples, and that column C is a measure of the open chromatin in the controls. It's also clear that values of 8 or so are larger than values around 0, and since these are logged data, it's somewhere around 32 256-times larger in disease than control. So yes, the data in your table indicate that there is open chromatin in the diseased samples and probably no open chromatin in your controls.

The other reason it looks like you are trolling is that all of the genes in your table except for that lincRNA are on chrY. The lincRNA is on chr6, but not in the region you are showing.