I am new to RNA-seq analysis. I have finished RNA alignment using
STAR, and got
ReadsPerGene.out.tab outputs. I am trying to use
tximport to build a gene-level count matrix for DESeq2 analysis using the STAR outputs. Yet I haven't been able to find a way to do it. I have checked the vignettes for tximport and DESeq2.
I am wondering if anyone has suggestions on how to use tximport and STAR outputs to make a gene-level count matrix?