Hi, I am trying to rational target selection from my data mining work. So far most of my analysis was done with limma, and I came across a published study where the group shared their result generated from DESeq2. and the questions are:

1) How translatable are the DEG results calculated from limma and DESeq2?

2) is it appropriate if I pool the DEGs identified with DESeq2 or limma together as my discovered gene target? or, should I reanalyze the DESeq2 data with limma?

Thanks in advance for the help. LilyC

I don't see any reason why you would need to re-analyse all the datasets using limma. DESeq2 and limma are both well regarded methods.

Whether it is reasonable to pool DEGs from two different experiments depends on how comparable the two experiments are from a biological point of view rather than on which statistical test was used for each analysis.