Perfoming clumping using ieugwasr package
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1
Entering edit mode
@1b588f00
Last seen 3 months ago
Germany

I am conducting a mendelian randomization study, and as an exposure I have hundreds of triats. The code below is part of a loop that iterates over these triats.


  # Apply Clumping 
  SeqId_data <- ieugwasr::ld_clump_local (
  SeqId_data,
  clump_p = 1,
  clump_r2 = r2,
  clump_kb = 10000,
  plink_bin = genetics.binaRies::get_plink_binary(), 
  bfile= "/path/to/reference/EUR" )

And am getting this output

[1] "1 10001_7"
[1] "Loading dataset with the following SeqId: 10008_43"
PLINK v1.90b6.21 64-bit (19 Oct 2020)          www.cog-genomics.org/plink/1.9/
(C) 2005-2020 Shaun Purcell, Christopher Chang   GNU General Public License v3
Logging to /tmp/RtmphPuzvw/file12b415f4a3e.log.
Options in effect:
  --bfile /home/a/alayash/TestFunctions/Sig1/EUR
  --clump /tmp/RtmphPuzvw/file12b415f4a3e
  --clump-kb 10000
  --clump-p1 1
  --clump-r2 0.099
  --out /tmp/RtmphPuzvw/file12b415f4a3e

132236 MB RAM detected; reserving 66118 MB for main workspace.
8550156 variants loaded from .bim file.
503 people (0 males, 0 females, 503 ambiguous) loaded from .fam.
Ambiguous sex IDs written to /tmp/RtmphPuzvw/file12b415f4a3e.nosex .
Using 1 thread (no multithreaded calculations invoked).
Before main variant filters, 503 founders and 0 nonfounders present.
Calculating allele frequencies... done.
8550156 variants and 503 people pass filters and QC.
Note: No phenotypes present.
Warning: No significant --clump results.  Skipping.
Error in file(file, "rt") : cannot open the connection
In addition: Warning message:
In file(file, "rt") :
  cannot open file '/tmp/RtmphPuzvw/file12b415f4a3e.clumped': No such file or directory

Any idea what might be the problem?

Thank you

LinkageDisequilibrium genom linka GenomicDistributionsData • 680 views
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Entering edit mode
@james-w-macdonald-5106
Last seen 7 hours ago
United States

Plink said

Warning: No significant --clump results.  Skipping.

And then R said it can't open the results file from running Plink. Which won't exist, because Plink already said there were none.

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