Why library sizes in the DGE object are different from original library sizes? And which library sizes should be used in differential expression analysis?
No I didn't filter the low expressed genes. I just created the DGE object and checked the library sizes within it and found them to be different from the library sizes in the metadata. So, I am wondering why the library sizes are different. What happens to them when creating a DGE object?
No I didn't filter the low expressed genes. I just created the DGE object and checked the library sizes within it and found them to be different from the library sizes in the metadata. So, I am wondering why the library sizes are different. What happens to them when creating a DGE object?
I just showed you that creating the
DGEList
object doesn't change the library sizes. You can try running my code to prove it to yourself.