Hello,

I am using DEseq2 to analyze the relationship between gene expression and two continuous phenotypic predictor variables, one of which has both positive and negative values. Is this acceptable, or does this variable need to be transformed so that all values are positive?

Thanks

Code should be placed in three backticks as shown below

# include your problematic code here with any corresponding output 
# please also include the results of running the following in an R session 

sessionInfo( )

Not a problem.

However, if the values are very large (eg 1e6) or very small (eg 1e-6), it's a good idea to center and scale the variable, with dds$x <- (dds$x - mean(dds$x))/sd(dds$x)